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Peer-Reviewed Publications

Immunological Identification of Fungal Species.

Nogueira F, Istel F, Pereira L, Tscherner M, Kuchler K

Methods Mol Biol. 2017;1508:339-359

Abstract Immunodetection is described in this chapter as a technique for producing specific antibodies for antigen detection of the major human fungal pathogens. In the case of Candida spp., heat-killed cells are used to immunize mice over a couple of weeks and then splenocytes are isolated and further fused with myelomas to easily propagate the antibodies produced in the mice. The resulting antibodies follow a purification process where antibody levels and concentrations are determined. Fungal cells are also lysed to obtain whole cell extracts as a prior step for identification of antigens using immunoprecipitation. Finally, this method permits the production of specific antibodies against fungi and the identification of the respective antigens in an in vivo model.
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PMID: 27837515 [PubMed - indexed for MEDLINE]

Peer-Reviewed Publications authored by Karl Kuchler from Pubmed Logo at the NCBI.
 
Karl Kuchler Laboratory, Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry,
Dr. Bohr-Gasse 9/2, A-1030 Vienna, AUSTRIA, [T] +43/1/4277-61806, [F] +43/1/4277-9618, [E] karl.kuchler@meduniwien.ac.at