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Peer-Reviewed Publications

Remodeling of the Candida glabrata cell wall in the gastrointestinal tract affects the gut microbiota and the immune response.

Charlet R, Pruvost Y, Tumba G, Istel F, Poulain D, Kuchler K, Sendid B, Jawhara S

Sci Rep. 2018 Feb 20;8(1):3316

Abstract The gastrointestinal (GI) microbiota acts a natural barrier to the proliferation of opportunistic pathogens. Candida glabrata is an opportunistic yeast pathogen that has adapted to colonize all segments of the human GI tract. We observed an increase in Escherichia coli, Enterococcus faecalis, and Bacteroides vulgatus populations, and a decrease in Lactobacillus johnsonii, Bacteroides thetaiotaomicron, and Bifidobacterium animalis in mice with DSS-induced colitis. This reduction was more pronounced for L. johnsonii during C. glabrata overgrowth. In addition, C. glabrata overgrowth increased mouse mortality and inflammatory parameters, and modulated the expression of intestinal receptors and signaling pathways. The C. glabrata cell wall underwent various changes during the course of C. glabrata colonization, and showed a significant increase in chitin. C. glabrata deficient in chitin synthase-3 induced fewer inflammatory parameters than the parental strain during intestinal inflammation. Oral administration of chitin attenuated the impact of colitis, and reduced the number of aerobic bacteria and C. glabrata overgrowth, while chitinase-3-like protein-1 increased. This study provides evidence that inflammation of the gut alters the microbial balance and leads to C. glabrata cell wall remodeling through an increase in chitin, which is involved in promoting persistence of C. glabrata in the gut.
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PMID: 29463799 [PubMed - in process]

Peer-Reviewed Publications authored by Karl Kuchler from Pubmed Logo at the NCBI.
 
Karl Kuchler Laboratory, Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry,
Dr. Bohr-Gasse 9/2, A-1030 Vienna, AUSTRIA, [T] +43/1/4277-61806, [F] +43/1/4277-9618, [E] karl.kuchler@meduniwien.ac.at